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Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response.
| Title | Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response. |
| Publication Type | Journal Article |
| Year of Publication | 1994 |
| Authors | McGuire, TC, Stephens, EB, Palmer, GH, McElwain, TF, Lichtensteiger, CA, Leib, SR, Barbet, AF |
| Journal | Vaccine |
| Volume | 12 |
| Issue | 5 |
| Pagination | 465-71 |
| Date Published | 1994 Apr |
| ISSN | 0264-410X |
| Keywords | Anaplasma, Animals, Antibodies, Bacterial, Antigens, Bacterial, Cell Line, Cercopithecus aethiops, Enzyme-Linked Immunosorbent Assay, Genes, Bacterial, Glycosylphosphatidylinositols, Mice, Mice, Inbred BALB C, Promoter Regions, Genetic, Protein Sorting Signals, Recombinant Fusion Proteins, Vaccinia virus |
| Abstract | Anaplasma marginale surface protein MSP-1a was expressed by recombinant vaccinia viruses with different promoters and as hybrid proteins. Transcription of msp1 alpha with P11 late promoter resulted in more MSP-1a than with P7.5 early-late promoter; however, mice immunized with the recombinants had similar antibody titres. Recombinants expressing hybrid MSP-1a with either a murine leukaemia virus or a trypanosomal glycoprotein signal sequence did not enhance antibody responses and resulted in a diffuse intracellular distribution of MSP-1a which did not accumulate in the Golgi apparatus as was noted in the absence of these signal sequences. In contrast, antibody titres to MSP-1a in mice immunized with a recombinant virus expressing hybrid MSP-1a with a trypanosomal GPI anchor signal sequence were significantly increased over all other constructs. |
| Alternate Journal | Vaccine |
| PubMed ID | 8023555 |
