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Home ยป Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.

Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.

TitleDetection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.
Publication TypeJournal Article
Year of Publication1991
AuthorsTrueblood, ES, McGuire, TC, Palmer, GH
JournalJ Clin Microbiol
Volume29
Issue7
Pagination1542-4
Date Published1991 Jul
ISSN0095-1137
KeywordsAnaplasmosis, Animals, Antibodies, Monoclonal, Antigens, Bacterial, Cattle, Cattle Diseases, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Sensitivity and Specificity, Sepsis
Abstract

An antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the Anaplasma marginale MSP1a surface protein. The assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. Positive tests preceded the onset of anemia by a mean of 2 days. The assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasitic pathogens.
Alternate JournalJ. Clin. Microbiol.
PubMed ID1885753
PubMed Central IDPMC270153