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Demonstration of heterogeneity among the antigenic proteins of Mobiluncus species.
Title | Demonstration of heterogeneity among the antigenic proteins of Mobiluncus species. |
Publication Type | Journal Article |
Year of Publication | 1990 |
Authors | Schwebke, JR, Hillier, SL, Fohn, MJ, Lukehart, SA |
Journal | J Clin Microbiol |
Volume | 28 |
Issue | 3 |
Pagination | 463-8 |
Date Published | 1990 Mar |
ISSN | 0095-1137 |
Keywords | Animals, Antibodies, Monoclonal, Antibody Specificity, Antigens, Bacterial, Bacteria, Anaerobic, Bacterial Infections, Bacterial Proteins, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Epitopes, Female, Fluorescent Antibody Technique, Humans, Immune Sera, Immunoblotting, Male, Rabbits, Species Specificity, Vaginitis |
Abstract | The protein and antigenic profiles of the American Type Culture Collection type strains of Mobiluncus species and those of 114 clinical isolates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and immunoblotting with homologous polyvalent antisera. The majority of isolates (82%) possessed characteristic protein profiles and could be identified to the species level by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The major protein bands were also antigenic, and some antigenic cross-reactivity was noted between the two Mobiluncus species. All of the isolates were examined for reactivity with a panel of 12 monoclonal antibodies previously prepared against the type strains. While 56 of 60 clinical isolates of Mobiluncus curtisii (93%) reacted with one or more of the monoclonal antibodies, only 23 of 54 clinical isolates which were identified as Mobiluncus mulieris by biochemical methods (48%) reacted with one or more of the monoclonal antibodies. One of the 4 M. curtisii isolates (25%) and 11 of the 31 M. mulieris isolates (35%) which did not react with the monoclonal antibodies also had atypical protein profiles. These results demonstrate a high degree of heterogeneity in the protein and antigenic profiles of Mobiluncus isolates and suggest that further taxonomic division may be appropriate. |
Alternate Journal | J. Clin. Microbiol. |
PubMed ID | 1691207 |
PubMed Central ID | PMC269645 |
Grant List | AI 07140-12 / AI / NIAID NIH HHS / United States AI 12192 / AI / NIAID NIH HHS / United States |