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Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus.
Title | Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Rose, TM, Ryan, JT, Schultz, ER, Raden, BW, Tsai, C-C |
Journal | J Virol |
Volume | 77 |
Issue | 9 |
Pagination | 5084-97 |
Date Published | 2003 May |
ISSN | 0022-538X |
Keywords | Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Directed DNA Polymerase, Evolution, Molecular, Herpesviridae Infections, Herpesvirus 8, Human, Humans, Macaca mulatta, Macaca nemestrina, Molecular Sequence Data, Monkey Diseases, Phylogeny, Retroperitoneal Fibrosis, Retroperitoneal Neoplasms, Rhadinovirus, Sarcoma, Kaposi, Sequence Analysis, DNA, Tumor Virus Infections |
Abstract | We previously identified retroperitoneal fibromatosis-associated herpesvirus (RFHV) as a simian homolog of Kaposi's sarcoma-associated herpesvirus (KSHV) in a fibroproliferative malignancy of macaques that has similarities to Kaposi's sarcoma. In this report, we cloned 4.3 kb of divergent locus B (DL-B) flanking the DNA polymerase gene from two variants of RFHV from different species of macaque with a consensus degenerate hybrid oligonucleotide primer approach. Within the DL-B region of RFHV, viral homologs of the cellular interleukin-6, dihydrofolate reductase, and thymidylate synthase genes were identified, along with a homolog of the gammaherpesvirus open reading frame (ORF) 10. In addition, a homolog of the KSHV ORF K3, the modulator of immune recognition-1, was identified. Our data show a close similarity in sequence conservation, gene content, and genomic structure between RFHV and KSHV which strongly supports the grouping of these viral species within the same RV-1 rhadinovirus lineage and the hypothesis that RFHV is the macaque homolog of KSHV. |
Alternate Journal | J. Virol. |
PubMed ID | 12692211 |
PubMed Central ID | PMC153986 |
Grant List | K02-AI49275 / AI / NIAID NIH HHS / United States R01-RR13154 / RR / NCRR NIH HHS / United States R55-CA72237 / CA / NCI NIH HHS / United States |