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Two new monoclonal antibodies for biochemical and flow cytometric analyses of human interferon regulatory factor-3 activation, turnover, and depletion.
Title | Two new monoclonal antibodies for biochemical and flow cytometric analyses of human interferon regulatory factor-3 activation, turnover, and depletion. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Rustagi, A, Doehle, BP, McElrath, JM, Gale, M |
Journal | Methods |
Volume | 59 |
Issue | 2 |
Pagination | 225-32 |
Date Published | 2013 Feb |
ISSN | 1095-9130 |
Abstract | Interferon regulatory factor-3 (IRF-3) is a master transcription factor that drives the host intracellular innate immune response to virus infection. The importance of IRF-3 in innate immune responses is highlighted by the fact that pathogenic viruses have developed strategies for antagonism of IRF-3. Several tools exist for evaluation of viral regulation of IRF-3 activation and function, but high-quality monoclonal antibodies that mark the differential activation states of human IRF-3 are lacking. To study IRF-3 activation, turnover, and depletion in a high-throughput manner in the context of virus infection, we have developed two new monoclonal antibodies to human IRF-3. These antibodies detect IRF-3 in virus-infected cells in a wide variety of assays and provide a new tool to study virus-host interactions and innate immune signaling. |
DOI | 10.1016/j.ymeth.2012.05.011 |
Alternate Journal | Methods |
PubMed ID | 22705311 |