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Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses.
Title | Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Mwangi, DM, Honda, Y, Graham, SP, Pelle, R, Taracha, ELN, Gachanja, J, Nyanjui, JK, Bray, J, Palmer, GH, Brown, WC, Mwangi, W |
Journal | Vet Immunol Immunopathol |
Volume | 140 |
Issue | 3-4 |
Pagination | 244-51 |
Date Published | 2011 Apr 15 |
ISSN | 1873-2534 |
Keywords | Adjuvants, Immunologic, Animals, Antigens, Protozoan, Cattle, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Granulocyte-Macrophage Colony-Stimulating Factor, Interferon-gamma, Lymphocyte Activation, Membrane Proteins, Protozoan Vaccines, Recombinant Proteins, T-Lymphocytes, Cytotoxic, Theileria parva, Theileriasis, Vaccines, DNA |
Abstract | Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4(+) and CD8(+) T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p<0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4(+) and CD8(+) T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses. |
DOI | 10.1016/j.vetimm.2010.12.013 |
Alternate Journal | Vet. Immunol. Immunopathol. |
PubMed ID | 21288576 |