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Transforming growth factor-beta 2: cDNA cloning and sequence analysis.
| Title | Transforming growth factor-beta 2: cDNA cloning and sequence analysis. |
| Publication Type | Journal Article |
| Year of Publication | 1988 |
| Authors | Madisen, L, Webb, NR, Rose, TM, Marquardt, H, Ikeda, T, Twardzik, D, Seyedin, S, Purchio, AF |
| Journal | DNA |
| Volume | 7 |
| Issue | 1 |
| Pagination | 1-8 |
| Date Published | 1988 Jan-Feb |
| ISSN | 0198-0238 |
| Keywords | Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Humans, Molecular Sequence Data, Peptides, Protein Precursors, Protein Processing, Post-Translational, Sequence Homology, Nucleic Acid, Transforming Growth Factors, Tumor Cells, Cultured |
| Abstract | We have obtained a cDNA clone coding for human transforming growth factor (TGF)-beta 2. The clone was isolated from a tamoxifen-treated human prostatic adenocarcinoma cell line (PC-3) using oligonucleotide probes based on the partial amino acid sequence of purified TGF-beta 2. The cDNA sequence predicts that TGF-beta 2 is synthesized as a 442-amino-acid polypeptide precursor from which the mature 112-amino-acid TGF-beta 2 subunit is derived by proteolytic cleavage. The proteins coded for by the human TGF-beta 1 and TGF-beta 2 cDNAs show an overall homology of 41%. The mature and amino-terminal precursor regions show 71% and 31% homology, respectively. Northern blot analysis identified TGF-beta 2 transcripts of 4.1, 5.1, and 6.5 kb using mRNA from several different sources. Analysis of polyadenylated RNA from tamoxifen-treated PC-3 cells showed that these cells contain higher numbers of transcripts for TGF-beta 1 than for TGF-beta 2, although they produce more TGF-beta 2 protein than TGF-beta 1. This suggests that there is a post-transcriptional level of regulation for the production of these proteins. |
| Alternate Journal | DNA |
| PubMed ID | 3162414 |
