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Stability and tick transmission phenotype of gfp-transformed Anaplasma marginale through a complete in vivo infection cycle.
Title | Stability and tick transmission phenotype of gfp-transformed Anaplasma marginale through a complete in vivo infection cycle. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Noh, SM, Ueti, MW, Palmer, GH, Munderloh, UG, Felsheim, RF, Brayton, KA |
Journal | Appl Environ Microbiol |
Volume | 77 |
Issue | 1 |
Pagination | 330-4 |
Date Published | 2011 Jan |
ISSN | 1098-5336 |
Keywords | Anaplasma marginale, Anaplasmosis, Animals, Cattle, Cattle Diseases, Disease Reservoirs, Disease Transmission, Infectious, Genes, Reporter, Green Fluorescent Proteins, Staining and Labeling, Ticks |
Abstract | We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp-transformed A. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformed A. marginale and the wild type were the same. However, there were significantly lower levels of the transformed A. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission. |
DOI | 10.1128/AEM.02096-10 |
Alternate Journal | Appl. Environ. Microbiol. |
PubMed ID | 21057014 |
PubMed Central ID | PMC3019711 |
Grant List | GR075800M / / Wellcome Trust / United Kingdom R01 AI 44005 / AI / NIAID NIH HHS / United States R37 AI044005-14 / AI / NIAID NIH HHS / United States |