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Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs.
Title | Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs. |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | Chandran, B, Smith, MS, Koelle, DM, Corey, L, Horvat, R, Goldstein, E |
Journal | Virology |
Volume | 243 |
Issue | 1 |
Pagination | 208-17 |
Date Published | 1998 Mar 30 |
ISSN | 0042-6822 |
Keywords | Acquired Immunodeficiency Syndrome, Antibodies, Viral, Antibody Specificity, Antigens, Viral, Cell Line, DNA, Complementary, Fluorescent Antibody Technique, Glycoproteins, Herpesvirus 8, Human, Humans, Male, Radioimmunoassay, Sarcoma, Kaposi, Viral Proteins |
Abstract | The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only low levels of HHV-8 antibodies were detected in the seropositive sera. In contrast, high levels of antibodies against HHV-8 lytic and latent antigens were detected by IFA in the sera from HIV+ Kaposi's sarcoma (KS)-positive individuals. These sera recognized several proteins and glycoproteins from BCBL-1 cells in RIP reactions. Two types of antibody responses were detected in the sera from HIV+ KS- homosexual men. In majority of the sera with and without detectable HHV-8 DNA in the peripheral blood mononuclear cells (PBMC), significantly low levels of HHV-8 antibodies were detected by IFA. These sera recognized only a subset of HHV-8 proteins and glycoproteins in RIP reactions. In contrast, in a subgroup of sera from HIV+ KS- homosexual men, higher levels of IFA antibodies against HHV-8 lytic and latent antigens were detected. These sera also recognized several viral proteins and glycoproteins in RIP reactions. These results suggest that antibody response profiles to HHV-8 infection vary significantly and serologic assays to detect antibody responses to a panel of both lytic and latent antibodies may be required for maximum sensitivity. Screening of a cDNA library from TPA-induced BCBL-1 cells with an HIV+ KS+ serum identified cDNAs encoding 12 HHV-8 proteins. Further characterization of these HHV-8 proteins would define the HHV-8 antigens useful for seroepidemiological studies and in discriminating lytic, latent, past, and/or reactivation infections. |
DOI | 10.1006/viro.1998.9055 |
Alternate Journal | Virology |
PubMed ID | 9527930 |