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Home ยป Rapid fluorescence-based measurement of neutrophil migration in vitro.

Rapid fluorescence-based measurement of neutrophil migration in vitro.

TitleRapid fluorescence-based measurement of neutrophil migration in vitro.
Publication TypeJournal Article
Year of Publication1998
AuthorsFrevert, CW, Wong, VA, Goodman, RB, Goodwin, R, Martin, TR
JournalJ Immunol Methods
Volume213
Issue1
Pagination41-52
Date Published1998 Apr 1
ISSN0022-1759
KeywordsCell Count, Chemotaxis, Leukocyte, Evaluation Studies as Topic, Fluoresceins, Fluorescence, Fluorescent Dyes, Humans, Micropore Filters, Neutrophils, Polycarboxylate Cement, Polyesters, Spectrometry, Fluorescence, Time Factors
Abstract

We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.
Alternate JournalJ. Immunol. Methods
PubMed ID9671124
Grant ListAI29103 / AI / NIAID NIH HHS / United States
HL30542 / HL / NHLBI NIH HHS / United States
HL51072 / HL / NHLBI NIH HHS / United States