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Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma.
Title | Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Hermouet, S, Sutton, CA, Rose, TM, Greenblatt, RJ, Corre, I, Garand, R, Neves, AM, Bataille, R, Casey, JW |
Journal | Leukemia |
Volume | 17 |
Issue | 1 |
Pagination | 185-95 |
Date Published | 2003 Jan |
ISSN | 0887-6924 |
Keywords | Adolescent, Adult, Aged, Amino Acid Sequence, Base Sequence, Burkitt Lymphoma, Case-Control Studies, Child, Child, Preschool, Cytomegalovirus, DNA Primers, DNA, Viral, Female, Herpesvirus 4, Human, Herpesvirus 6, Human, Herpesvirus 7, Human, Herpesvirus 8, Human, Humans, Infant, Leukemia, Lymphocytic, Chronic, B-Cell, Male, Middle Aged, Molecular Sequence Data, Multiple Myeloma, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Viral Load |
Abstract | Real-time quantitative polymerase chain reaction (qPCR) was used to quantify viral loads of human herpesviruses (HHVs) at diagnosis in 61 samples of malignant B cells: 21 chronic lymphocytic leukemia (B-CLL), 29 acute lymphoblastic leukemia (B-ALL) and 11 multiple myeloma (MM); control samples were blasts from 16 acute myeloid leukemia (AML) and 24 blood or bone marrow samples from healthy donors. The majority of samples from healthy donors and patients (B-ALL, B-CLL or AML, but not MM) was positive for EBV and contained <100 ebv copies/100 ng dna. ebv loads were occasionally high (>500 copies/100 ng DNA) in B-ALL (2/16) and in B-CLL (2/21) samples. The fractions of samples positive for HHV-8 and HHV-6A, less than 10% for MM patients, were high for adults with B-ALL (18.8% HHV-8+, 43.8% HHV-6A+) or B-CLL (28.6% HHV-8+, 52.4% HHV-6A+). B-ALL, B-CLL and MM samples were rarely positive for HHV-6B and HHV-7. Lastly, 75% of B-ALL samples were positive for CMV, and CMV loads were significantly higher in B-ALL samples than in MM, B-CLL or AML samples. We also used PCR with consensus-degenerate hybrid oligonucleotide primers (CODEHOP) to look for novel HHVs in B cell samples: no sequence indicative of a new HHV was detected. Altogether, the data indicate that the presence of multiple HHVs, including EBV and CMV at high loads, is not rare in B-ALL and B-CLL cell samples. Future prospective studies should determine whether patients with high EBV/CMV loads at diagnosis in tumor samples face a higher risk of delayed hematological recovery, virus-related complications or relapse. |
DOI | 10.1038/sj.leu.2402748 |
Alternate Journal | Leukemia |
PubMed ID | 12529677 |
Grant List | T2ES07052E / ES / NIEHS NIH HHS / United States |