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Proteomic analysis of bronchoalveolar lavage fluid proteins from mice infected with Francisella tularensis ssp. novicida.
Title | Proteomic analysis of bronchoalveolar lavage fluid proteins from mice infected with Francisella tularensis ssp. novicida. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Varnum, SM, Webb-Robertson, B-JM, Pounds, JG, Moore, RJ, Smith, RD, Frevert, CW, Skerrett, SJ, Wunschel, D |
Journal | J Proteome Res |
Volume | 11 |
Issue | 7 |
Pagination | 3690-703 |
Date Published | 2012 Jul 6 |
ISSN | 1535-3907 |
Keywords | Acute-Phase Proteins, Animals, Bronchoalveolar Lavage Fluid, Complement System Proteins, Francisella tularensis, Immunity, Innate, Male, Mice, Mice, Inbred C57BL, Neutrophils, Oxidative Stress, Proteome, Proteomics, Pseudomonas aeruginosa, Pseudomonas Infections, Tularemia |
Abstract | Francisella tularensis causes the zoonosis tularemia in humans and is one of the most virulent bacterial pathogens. We utilized a global proteomic approach to characterize protein changes in bronchoalveolar lavage fluid from mice exposed to one of three organisms, F. tularensis ssp. novicida, an avirulent mutant of F. tularensis ssp. novicida (F.t. novicida-ΔmglA), and Pseudomonas aeruginosa. The composition of bronchoalveolar lavage fluid (BALF) proteins was altered following infection, including proteins involved in neutrophil activation, oxidative stress, and inflammatory responses. Components of the innate immune response were induced including the acute phase response and the complement system; however, the timing of their induction varied. F. tularensis ssp. novicida infected mice do not appear to have an effective innate immune response in the first hours of infection; however, within 24 h, they show an upregulation of innate immune response proteins. This delayed response is in contrast to P. aeruginosa infected animals which show an early innate immune response. Likewise, F.t. novicida-ΔmglA infection initiates an early innate immune response; however, this response is diminished by 24 h. Finally, this study identifies several candidate biomarkers, including Chitinase 3-like-1 (CHI3L1 or YKL-40) and peroxiredoxin 1, that are associated with F. tularensis ssp. novicida but not P. aeruginosa infection. |
DOI | 10.1021/pr3001767 |
Alternate Journal | J. Proteome Res. |
PubMed ID | 22663564 |
PubMed Central ID | PMC3423085 |
Grant List | 3U54AI057141 / AI / NIAID NIH HHS / United States HL098067 / HL / NHLBI NIH HHS / United States P01 HL098067 / HL / NHLBI NIH HHS / United States P41 RR018522 / RR / NCRR NIH HHS / United States R21 RR030249 / RR / NCRR NIH HHS / United States RR030249 / RR / NCRR NIH HHS / United States RR18522 / RR / NCRR NIH HHS / United States |