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Protective immunity induced by immunization with a live, cultured Anaplasma marginale strain.
Title | Protective immunity induced by immunization with a live, cultured Anaplasma marginale strain. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | G Hammac, K, Ku, P-S, Galletti, MF, Noh, SM, Scoles, GA, Palmer, GH, Brayton, KA |
Journal | Vaccine |
Volume | 31 |
Issue | 35 |
Pagination | 3617-22 |
Date Published | 2013 Aug 2 |
ISSN | 1873-2518 |
Abstract | Despite significant economic losses resulting from infection with Anaplasma marginale, a tick-transmitted rickettsial pathogen of cattle, available vaccines provide, at best, only partial protection against clinical disease. The green-fluorescent protein expressing mutant of the A. marginale St. Maries strain is a live, marked vaccine candidate (AmStM-GFP). To test whether AmStM-GFP is safe and provides clinical protection, a group of calves was vaccinated, and clinical parameters, including percent parasitized erythrocytes (PPE), packed cell volume (PCV) and days required to reach peak bacteremia, were measured following inoculation and following tick challenge with wild type St. Maries strain (AmStM). These clinical parameters were compared to those obtained during infection with the A. marginale subsp. centrale vaccine strain (A. centrale) or wild type AmStM. AmStM-GFP resulted in similar clinical parameters to A. centrale, but had a lower maximum PPE, smaller drop in PCV and took longer to reach peak bacteremia than wild type AmStM. AmStM-GFP provided clinical protection, yielding a stable PCV and low bacteremia following challenge, whereas A. centrale only afforded partial clinical protection. |
DOI | 10.1016/j.vaccine.2013.04.069 |
Alternate Journal | Vaccine |
PubMed ID | 23664994 |
Grant List | GR075800M / / Wellcome Trust / United Kingdom R01 AI044005 / AI / NIAID NIH HHS / United States R01 AI44005 / AI / NIAID NIH HHS / United States T32 AI007025 / AI / NIAID NIH HHS / United States T32AI007025 / AI / NIAID NIH HHS / United States |