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Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron.
Title | Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Deriu, E, Liu, JZ, Pezeshki, M, Edwards, RA, Ochoa, RJ, Contreras, H, Libby, SJ, Fang, FC, Raffatellu, M |
Journal | Cell Host Microbe |
Volume | 14 |
Issue | 1 |
Pagination | 26-37 |
Date Published | 2013 Jul 17 |
ISSN | 1934-6069 |
Abstract | Host inflammation alters the availability of nutrients such as iron to limit microbial growth. However, Salmonella enterica serovar Typhimurium thrives in the inflamed gut by scavenging for iron with siderophores. By administering Escherichia coli strain Nissle 1917, which assimilates iron by similar mechanisms, we show that this nonpathogenic bacterium can outcompete and reduce S. Typhimurium colonization in mouse models of acute colitis and chronic persistent infection. This probiotic activity depends on E. coli Nissle iron acquisition, given that mutants deficient in iron uptake colonize the intestine but do not reduce S. Typhimurium colonization. Additionally, the ability of E. coli Nissle to overcome iron restriction by the host protein lipocalin 2, which counteracts some siderophores, is essential, given that S. Typhimurium is unaffected by E. coli Nissle in lipocalin 2-deficient mice. Thus, iron availability impacts S. Typhimurium growth, and E. coli Nissle reduces S. Typhimurium intestinal colonization by competing for this limiting nutrient. |
DOI | 10.1016/j.chom.2013.06.007 |
Alternate Journal | Cell Host Microbe |
PubMed ID | 23870311 |
PubMed Central ID | PMC3752295 |
Grant List | AI083663 / AI / NIAID NIH HHS / United States AI77629 / AI / NIAID NIH HHS / United States R01 AI077629 / AI / NIAID NIH HHS / United States R01 AI083663 / AI / NIAID NIH HHS / United States T32 AI060573 / AI / NIAID NIH HHS / United States T32 AI60573 / AI / NIAID NIH HHS / United States U54AI065359 / AI / NIAID NIH HHS / United States |