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PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica.
Title | PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Navarre, WWiley, S Zou, B, Roy, H, Xie, JLucy, Savchenko, A, Singer, A, Edvokimova, E, Prost, LR, Kumar, R, Ibba, M, Fang, FC |
Journal | Mol Cell |
Volume | 39 |
Issue | 2 |
Pagination | 209-21 |
Date Published | 2010 Jul 30 |
ISSN | 1097-4164 |
Keywords | Animals, Bacterial Proteins, Drug Resistance, Microbial, Female, Gene Expression Regulation, Bacterial, Genomic Islands, Lysine, Lysine-tRNA Ligase, Mice, Peptide Elongation Factors, Protein Processing, Post-Translational, Salmonella enterica, Virulence Factors |
Abstract | We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA. |
DOI | 10.1016/j.molcel.2010.06.021 |
Alternate Journal | Mol. Cell |
PubMed ID | 20670890 |
PubMed Central ID | PMC2913146 |
Grant List | AI039557 / AI / NIAID NIH HHS / United States AI050660 / AI / NIAID NIH HHS / United States GM065183 / GM / NIGMS NIH HHS / United States MOP-86683 / / Canadian Institutes of Health Research / Canada R01 AI039557-04 / AI / NIAID NIH HHS / United States R01 AI050660-05 / AI / NIAID NIH HHS / United States R01 GM065183-09 / GM / NIGMS NIH HHS / United States |