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A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber.
Title | A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Keenan, TM, Frevert, CW, Wu, A, Wong, V, Folch, A |
Journal | Lab Chip |
Volume | 10 |
Issue | 1 |
Pagination | 116-22 |
Date Published | 2010 Jan 7 |
ISSN | 1473-0197 |
Keywords | Cells, Cultured, Chemotactic Factors, Chemotaxis, Fluorescent Dyes, Humans, Microfluidic Analytical Techniques, Neutrophil Infiltration, Neutrophils |
Abstract | During inflammation neutrophils rapidly migrate to the site of tissue damage or infection by following complex gradients of bacterial peptides and host-derived chemokines. The efficiency and speed of neutrophil migration is critically dependent upon the ability of neutrophils to sense new gradients and utilize only those that provide the most direct path to the damaged or infected site. Receptor desensitization plays an important role in migration efficiency and is most commonly studied using bath application of chemotactic factor solutions instead of presenting cells with gradients analogous to those they would experience in vivo. Here we describe a new method for examining gradient-induced neutrophil desensitization using a previously-developed open-chamber microfluidic gradient generator. |
DOI | 10.1039/b913494h |
Alternate Journal | Lab Chip |
PubMed ID | 20024059 |
PubMed Central ID | PMC3786699 |
Grant List | R01 NS064387 / NS / NINDS NIH HHS / United States R33 EB003307-03 / EB / NIBIB NIH HHS / United States |