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New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae.
Title | New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Soge, OO, Adeniyi, BA, Roberts, MC |
Journal | J Antimicrob Chemother |
Volume | 58 |
Issue | 5 |
Pagination | 1048-53 |
Date Published | 2006 Nov |
ISSN | 0305-7453 |
Keywords | Base Sequence, beta-Lactamases, Community-Acquired Infections, Drug Resistance, Drug Resistance, Bacterial, Humans, Klebsiella Infections, Klebsiella pneumoniae, Microbial Sensitivity Tests, Nigeria, Plasmids |
Abstract | OBJECTIVES: To determine antibiotic resistance genes associated with 17 Nigerian CTX-M-positive Klebsiella pneumoniae plasmids from patients with community-acquired urinary tract infections. METHODS: The size and restriction patterns of the plasmids were determined, and antibiotic resistance genes were identified using DNA-DNA hybridization, PCR assays, hybridization of PCR products with internal probes, and sequencing. RESULTS: All CTX-M plasmids were large (58-320 kb) and carried the following genes: aac(6')-Ib (aminoglycoside resistance) which included aac(6')-Ib-cr (aminoglycoside-fluoroquinolone resistance), aadA2 (aminoglycoside resistance), erm(B) (macrolide-lincosamide-streptogramin B resistance), blaTEM-1 (ampicillin resistance), tet(A) (tetracycline resistance), sul1 (sulphonamide resistance), dfr (trimethoprim resistance) and intI1, an integrase associated with class 1 integrons. Eleven (65%) plasmids carried an mph(A) gene (macrolide resistance), seven (41%) plasmids carried a qnrB1 gene (low-level quinolone resistance) and four (24%) plasmids carried multiple cat genes (chloramphenicol resistance). catA2, catA3 and qnrB1 genes and a 6 kb PstI fragment, carrying the blaCTX-M gene, were sequenced. CONCLUSIONS: This is the first description of catA2 and catA3 genes in Klebsiella spp. and the first description of the erm(B) and floR genes associated with a CTX-M plasmid. This is also the first report of qnrB1 and aac(6')-Ib-cr in isolates from Africa and the first report of these two genes on the same plasmid. |
DOI | 10.1093/jac/dkl370 |
Alternate Journal | J. Antimicrob. Chemother. |
PubMed ID | 16997844 |