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The murine intravaginal HSV-2 challenge model for investigation of DNA vaccines.
Title | The murine intravaginal HSV-2 challenge model for investigation of DNA vaccines. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Marshak, JO, Dong, L, Koelle, DM |
Journal | Methods Mol Biol |
Volume | 1144 |
Pagination | 305-27 |
Date Published | 2014 |
ISSN | 1940-6029 |
Abstract | DNA vaccines have been licensed in veterinary medicine and have promise for humans. This format is relatively immunogenic in mice and guinea pigs, the two principle HSV-2 animal models, permitting rapid assessment of vectors, antigens, adjuvants, and delivery systems. Limitations include the relatively poor immunogenicity of naked DNA in humans and the profound differences in HSV-2 pathogenesis between host species. Herein, we detail lessons learned over the last few years investigating candidate DNA vaccines in the progesterone-primed female mouse vaginal model of HSV-2 infection as a guide to investigators in the field. |
DOI | 10.1007/978-1-4939-0428-0_21 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 24671693 |