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Macrophage-mediated killing of opsonized Treponema pallidum.
Title | Macrophage-mediated killing of opsonized Treponema pallidum. |
Publication Type | Journal Article |
Year of Publication | 1992 |
Authors | Baker-Zander, SA, Lukehart, SA |
Journal | J Infect Dis |
Volume | 165 |
Issue | 1 |
Pagination | 69-74 |
Date Published | 1992 Jan |
ISSN | 0022-1899 |
Keywords | Animals, Cells, Cultured, Fluorescent Antibody Technique, Humans, Immune Sera, Immunoblotting, Macrophages, Male, Opsonin Proteins, Phagocytosis, Rabbits, Treponema pallidum |
Abstract | The ability of proteose peptone-induced normal rabbit peritoneal macrophages to kill Treponema pallidum subspecies pallidum in vitro is demonstrated. Treponemes and 10% heated immune or normal sera were incubated with macrophages at a ratio of 1:200. After 2-10 h of incubation, these mixtures were injected intradermally at duplicate sites on normal rabbits. Maximal killing (failure to develop lesions) was seen at 10 h of incubation with immune serum: Only 7% (1/14) of lesions developed compared with 90% (9/10) after incubation in the presence of normal serum (P less than .001). Maximal phagocytosis (detected by immunofluorescence) occurred by 8 h in the presence of immune serum, when 90% of macrophages had ingested treponemes. At this point, however, 70% of lesion sites from macrophages incubated with treponemes and immune serum still developed, suggesting that effective killing may require at least 2 h after phagocytosis. |
Alternate Journal | J. Infect. Dis. |
PubMed ID | 1727898 |
Grant List | AI-18988 / AI / NIAID NIH HHS / United States |