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Laser cutting eliminates nucleic acid cross-contamination in dried-blood-spot processing.

TitleLaser cutting eliminates nucleic acid cross-contamination in dried-blood-spot processing.
Publication TypeJournal Article
Year of Publication2012
AuthorsMurphy, SC, Daza, G, Chang, M, Coombs, R
JournalJ Clin Microbiol
Volume50
Issue12
Pagination4128-30
Date Published2012 Dec
ISSN1098-660X
KeywordsBlood, Clinical Laboratory Techniques, Diagnostic Errors, DNA Contamination, Humans, Lasers, Malaria, Nucleic Acids, Specimen Handling
Abstract

Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.
DOI10.1128/JCM.02549-12
Alternate JournalJ. Clin. Microbiol.
PubMed ID23052309