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Human dendritic cells. Enrichment and characterization from peripheral blood.
Title | Human dendritic cells. Enrichment and characterization from peripheral blood. |
Publication Type | Journal Article |
Year of Publication | 1982 |
Authors | Van Voorhis, WC, Hair, LS, Steinman, RM, Kaplan, G |
Journal | J Exp Med |
Volume | 155 |
Issue | 4 |
Pagination | 1172-87 |
Date Published | 1982 Apr 1 |
ISSN | 0022-1007 |
Keywords | Animals, B-Lymphocytes, Cell Adhesion, Cell Separation, Endocytosis, Histocompatibility Antigens Class II, Humans, Lymphocyte Culture Test, Mixed, Lymphocytes, Lymphoid Tissue, Monocytes, Rabbits, Receptors, Antigen, B-Cell, Rosette Formation, T-Lymphocytes |
Abstract | Previous studies demonstrated that lymphoid tissues of mice and rats contain small numbers (less than 1 percent of nucleated cells) of dendritic cells (DC) with special cytologic, surface, and functional properties. We show here that similar DC represent 0.1-0.5 percent of human peripheral blood mononuclear cells. DC can be enriched to 20-60 percent purity by a multistep procedure analogous to that used in mice. Adherent peripheral blood mononuclear cells are cultured overnight, and the released cells are depleted of monocytes and B cells by readherence to plastic, rosetting with erythrocytes coated with anti-human IgG, and centrifugation in dense albumin columns. Enriched DC have similar cytologic features to rodent DC by light and electron microscopy. DC express HLA, and HLA-DR and the leukocyte-common antigens. They lack phagocytic capacity, receptors for antibody-coated and neuraminidase-treated erythrocytes, surface and intracellular Ig, esterase, peroxidase, and azurophilic granules. DC do not react with several monoclonal antibodies directed to phagocytes (OKM 1, "mac-1," 63D3, and 61D3) and T cells (OKT 3, 6, 8). Unlike the mouse, human DC express complement receptors. When maintained in culture for 4 d, human DC did not give rise to either B cells or monocytes. Therefore, DC identified by cytologic criteria are distinct from other leukocytes. Enriched populations of DC have been compared to fractions enriched in monocytes, B cells, and T cells in three functional assays: stimulation of the primary allogeneic mixed leukocyte reaction, stimulation of the primary syngeneic MLR, and accessory function for the proliferation of periodate- modified T cells. In each case, the DC fraction was 10-fold or more active than other cell fractions. We conclude that DC circulate in man, and represent the principal cell type required for the initiation of several immune responses. |
Alternate Journal | J. Exp. Med. |
PubMed ID | 6460832 |