You are here
HIV type 2 protease, reverse transcriptase, and envelope viral variation in the PBMC and genital tract of ARV-naive women in Senegal.
Title | HIV type 2 protease, reverse transcriptase, and envelope viral variation in the PBMC and genital tract of ARV-naive women in Senegal. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Gottlieb, GS, Hawes, SE, Wong, KG, Raugi, DN, Agne, HD, Critchlow, CW, Kiviat, NB, Sow, PSalif |
Journal | AIDS Res Hum Retroviruses |
Volume | 24 |
Issue | 6 |
Pagination | 857-64 |
Date Published | 2008 Jun |
ISSN | 1931-8405 |
Keywords | Adult, Amino Acid Sequence, Anti-HIV Agents, Cervix Uteri, Drug Resistance, Viral, env Gene Products, Human Immunodeficiency Virus, Female, HIV Infections, HIV Protease, HIV Reverse Transcriptase, HIV-2, Humans, Leukocytes, Mononuclear, Likelihood Functions, Middle Aged, Molecular Sequence Data, Mutation, Phylogeny, Polymorphism, Genetic, RNA, Viral, Senegal, Sequence Analysis, Protein, Vagina |
Abstract | Unique viral variants and resistance mutations may occur in the genital tract of HIV-2 ARV-naive infected women. We sequenced and phylogenetically analyzed protease (PR), reverse transcriptase (RT), and envelope (ENV) from PBMC and genital tract samples from four ARV-naive women in Senegal. HIV-2 protease polymorphisms that predict HIV-1 protease inhibitor (PI) resistance were common. Two subjects had protease mutations (T77I and I64V) in genital tract samples that were not found in PBMCs. One subject had the HIV-2 reverse transcriptase M184I mutation in CVL DNA (but not PBMCs) that is known to confer 3TC/FTC resistance in HIV-2. In another subject, the reverse transcriptase A62V mutation was also found in CVL-RNA but not PBMCs. We found no significant difference in ENV variants between PBMCs and the genital tract. HIV-2 RT and PR mutations in the genital tract of ARV-naive females may have implications for transmitted HIV-2 resistance and ARV therapy. |
DOI | 10.1089/aid.2008.0015 |
Alternate Journal | AIDS Res. Hum. Retroviruses |
PubMed ID | 18544024 |