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HIV gp120-specific cell-mediated immune responses in mice after oral immunization with recombinant Salmonella.
Title | HIV gp120-specific cell-mediated immune responses in mice after oral immunization with recombinant Salmonella. |
Publication Type | Journal Article |
Year of Publication | 1995 |
Authors | Berggren, RE, Wunderlich, A, Ziegler, E, Schleicher, M, Duke, RC, Looney, D, Fang, FC |
Journal | J Acquir Immune Defic Syndr Hum Retrovirol |
Volume | 10 |
Issue | 5 |
Pagination | 489-95 |
Date Published | 1995 Dec 15 |
ISSN | 1077-9450 |
Keywords | Administration, Oral, AIDS Vaccines, Animals, Antibodies, Bacterial, Base Sequence, DNA Primers, Enzyme-Linked Immunosorbent Assay, Female, HIV Antibodies, HIV Envelope Protein gp120, HIV Infections, HIV-1, Immunity, Cellular, Immunization, Intestinal Mucosa, Lymphocyte Activation, Male, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Plasmids, Salmonella typhimurium, T-Lymphocytes, Vaccines, Synthetic |
Abstract | Salmonella is of great interest as a potential human immunodeficiency virus vaccine vector because of its ability to elicit potent mucosal and systemic immune responses when administered orally. To determine whether such a vaccine could elicit an immune response in mice, plasmids expressing HIV gp120-LAI were introduced into attenuated S. typhimurium. Three serial doses of 10(10) recombinant organisms were administered orally to BALB/c mice at 2-week intervals. Immunized mice but not control mice demonstrated proliferative T cell responses to gp120-LAI, comparable in magnitude to the proliferative responses to Salmonella antigens. Immunized mice had detectable serum and intestinal Salmonella-specific IgA and serum Salmonella-specific IgG. However, no gp120-specific antibody was detected in either serum or intestinal washes. These results indicate that live recombinant Salmonella-based vaccine constructs can induce HIV-specific cellular immune responses in vivo. |
Alternate Journal | J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. |
PubMed ID | 8548327 |
Grant List | 1K08AI01248-01 / AI / NIAID NIH HHS / United States AI-07447-02 / AI / NIAID NIH HHS / United States AI-32463-03 / AI / NIAID NIH HHS / United States |