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Functional characterization of rat chemokine macrophage inflammatory protein-2.
| Title | Functional characterization of rat chemokine macrophage inflammatory protein-2. |
| Publication Type | Journal Article |
| Year of Publication | 1995 |
| Authors | Frevert, CW, Farone, A, Danaee, H, Paulauskis, JD, Kobzik, L |
| Journal | Inflammation |
| Volume | 19 |
| Issue | 1 |
| Pagination | 133-42 |
| Date Published | 1995 Feb |
| ISSN | 0360-3997 |
| Keywords | Animals, Base Sequence, Cell Adhesion Molecules, Cell Movement, Cells, Cultured, Chemokine CXCL2, Chemotactic Factors, Cytokines, Electrophoresis, Polyacrylamide Gel, L-Selectin, Macrophage-1 Antigen, Membrane Glycoproteins, Molecular Sequence Data, Monokines, Neutrophils, Oligonucleotide Probes, Rats, Respiratory Burst |
| Abstract | Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50 micrograms of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models. |
| Alternate Journal | Inflammation |
| PubMed ID | 7535749 |
| Grant List | ES 00002 / ES / NIEHS NIH HHS / United States HL02374 / HL / NHLBI NIH HHS / United States HL19170 / HL / NHLBI NIH HHS / United States |
