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Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells.
| Title | Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells. |
| Publication Type | Journal Article |
| Year of Publication | 2006 |
| Authors | Leader, BT, VanVoorhis, WC, Lukehart, SA |
| Journal | Vet Immunol Immunopathol |
| Volume | 113 |
| Issue | 3-4 |
| Pagination | 421-7 |
| Date Published | 2006 Oct 15 |
| ISSN | 0165-2427 |
| Keywords | Animals, B-Lymphocytes, Blotting, Western, Flow Cytometry, Histocompatibility Antigens Class II, Interleukin-4, Lymphocyte Activation, Male, Rabbits, Recombinant Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Spodoptera, T-Lymphocytes |
| Abstract | The purpose of the current study was to express recombinant rabbit IL-4 (rRbIL-4) and to characterize its biological activity. The cDNA of RbIL-4 was cloned into an insect cell expression vector that allowed for constitutive expression in Sf9 cells and incorporated a 6-histidine tag on the recombinant protein for purification. The purified protein corresponded to the predicted size of rRbIL-4 and was recognized by an anti-human IL-4 antibody in immunoblotting. As shown for IL-4 from other species, a dose-dependent proliferative response was observed in T-lymphoblasts cultured with rRbIL-4. rRbIL-4 also induced increased expression of MHC class II molecules on the surface of rabbit B-cells in a dose-dependent manner. These results indicate that we have produced recombinant rabbit IL-4 that exhibits expected biological activity on rabbit B and T-cells. |
| DOI | 10.1016/j.vetimm.2006.06.001 |
| Alternate Journal | Vet. Immunol. Immunopathol. |
| PubMed ID | 16879875 |
| Grant List | A1 34616 / / PHS HHS / United States A1 42143 / / PHS HHS / United States A1 43456 / / PHS HHS / United States A1 63940 / / PHS HHS / United States |
