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Distribution of mef(A) in gram-positive bacteria from healthy Portuguese children.
| Title | Distribution of mef(A) in gram-positive bacteria from healthy Portuguese children. |
| Publication Type | Journal Article |
| Year of Publication | 2002 |
| Authors | Luna, VA, Heiken, M, Judge, K, Ulep, C, van Kirk, N, Luis, H, Bernardo, M, Leitao, J, Roberts, MC |
| Journal | Antimicrob Agents Chemother |
| Volume | 46 |
| Issue | 8 |
| Pagination | 2513-7 |
| Date Published | 2002 Aug |
| ISSN | 0066-4804 |
| Keywords | Anti-Bacterial Agents, Bacterial Proteins, Child, DNA, Bacterial, Drug Resistance, Microbial, Genes, Bacterial, Gram-Positive Bacteria, Gram-Positive Bacterial Infections, Humans, Macrolides, Membrane Proteins, Molecular Sequence Data, Oligonucleotide Probes, Open Reading Frames, Phenotype, Portugal, Reverse Transcriptase Polymerase Chain Reaction, RNA |
| Abstract | We screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(A), erm(B), erm(C), erm(F), and mef(A) genes. The mef(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including Enterococcus spp., Staphylococcus spp., and Streptococcus spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn1207.1, a genetic element carrying mef(A) recently described in Streptococcus pneumoniae. The three Staphylococcus spp. did not carry orf1 to orf3. A gram-negative Acinetobacter junii isolate also carried the other seven ORFs described in Tn1207.1. A Staphylococcus aureus isolate, a Streptococcus intermedius isolate, a Streptococcus sp. isolate, and an Enterococcus sp. isolate had their mef(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the mef(A) gene from S. pneumoniae. |
| Alternate Journal | Antimicrob. Agents Chemother. |
| PubMed ID | 12121926 |
| PubMed Central ID | PMC127387 |
| Grant List | N01 DE-72623 / DE / NIDCR NIH HHS / United States T32 DE-07063 / DE / NIDCR NIH HHS / United States U01 DE 1189 / DE / NIDCR NIH HHS / United States |
