You are here
cDNA microarray analysis reveals fundamental differences in the expression profiles of primary human monocytes, monocyte-derived macrophages, and alveolar macrophages.
Title | cDNA microarray analysis reveals fundamental differences in the expression profiles of primary human monocytes, monocyte-derived macrophages, and alveolar macrophages. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Li, J, Pritchard, DK, Wang, X, Park, DR, Bumgarner, RE, Schwartz, SM, W Liles, C |
Journal | J Leukoc Biol |
Volume | 81 |
Issue | 1 |
Pagination | 328-35 |
Date Published | 2007 Jan |
ISSN | 0741-5400 |
Keywords | Adult, Biological Markers, Female, Gene Expression Profiling, Humans, Macrophages, Macrophages, Alveolar, Male, Monocytes, Oligonucleotide Array Sequence Analysis, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction |
Abstract | We report the systematic use of large-scale cDNA microarrays to study the gene expression profiles of primary human peripheral blood monocytes (MONO) in comparison with in vitro-differentiated, M-CSF-induced MONO-derived macrophages (MAC) and primary human alveolar MAC (AM), obtained by bronchoalveolar lavage from the lungs of normal volunteers. These studies revealed large-scale differences in the gene expression profile between both MAC types (MAC and AM) and MONO. In addition, large differences were observed in the gene expression profiles of the two MAC types. Specifically, 21% of genes on the array (2904 out of 13,582) were differentially expressed between AM and MONO, and 2229 out of 13,583 probes were differentially expressed between MAC and AM. Our expression data show remarkable differences in gene expression between different MAC subpopulations and emphasize the heterogeneity of different MAC populations. This study underscores the need to scrutinize models of MAC biology for relevance to specific disease processes. |
DOI | 10.1189/jlb.0206124 |
Alternate Journal | J. Leukoc. Biol. |
PubMed ID | 17046970 |