You are here
The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species.
| Title | The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species. |
| Publication Type | Journal Article |
| Year of Publication | 1992 |
| Authors | Visser, ES, McGuire, TC, Palmer, GH, Davis, WC, Shkap, V, Pipano, E, Knowles, DP |
| Journal | Infect Immun |
| Volume | 60 |
| Issue | 12 |
| Pagination | 5139-44 |
| Date Published | 1992 Dec |
| ISSN | 0019-9567 |
| Keywords | Amino Acid Sequence, Anaplasma, Animals, Antibodies, Monoclonal, Bacterial Outer Membrane Proteins, Base Sequence, Cattle, Cloning, Molecular, Epitopes, Genes, Bacterial, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Weight |
| Abstract | Immunization with Anaplasma marginale outer membranes induced immunity against clinical disease which correlated with antibody titer to outer membrane proteins, including a 19-kDa protein (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). This 19-kDa protein, designated major surface protein 5 (MSP-5), was encoded by a single-copy 633-bp gene. The molecular mass of MSP-5, defined in immunoblots by binding to monoclonal antibody ANAF16C1, was conserved among all recognized species of Anaplasma: A. marginale, A. centrale, and A. ovis. Recombinant MSP-5, which absorbed the antibody reactivity of bovine immune serum to native MSP-5, was recognized by anti-A. marginale and anti-A. centrale immune sera in a competitive inhibition assay with monoclonal antibody ANAF16C1. The presence of antibody to the epitope defined by monoclonal antibody ANAF16C1 in all postinfection sera tested indicates that this epitope is a potential diagnostic antigen for use in identifying persistently infected cattle. |
| Alternate Journal | Infect. Immun. |
| PubMed ID | 1280624 |
| PubMed Central ID | PMC258289 |
